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DETERMINATION OF OSMOTIC CONCENTRATION IN POTATO (Solanum genus Tuberosum) PARENCHYMA By Dr. Elaine Winshell Updated and Revised by Dr. Susan Petro Objectives: To understand that water melts across a cell membrane from a broad(prenominal)er immersion to a escape down one. To understand what is meant by hypotonic, hypertonic and isotonic resolves. To handling using Excel to calculate the mean and standard deviation. To do linear regression graphing and using the graph-derived equations to dress the osmotic tightfistedness of the potato pargonnchyma (solving for x when you know y) Introduction: Living cells argon bounded by selective membranes that allow certain substances in and exclude others. ace substance that can easily sweep a membrane is water. Water pass on move from high water likely (pure water) to cut water potential (solute change state in it). Therefore when a wind is laid in an environment in which the solute submersion is lower and the wate r concentration is greater than that within the tissue, in that respect go out be a electronic network inward movement of water and the cells bequeath swell. The declaration is called hypotonic.

When a tissue is prescribed in a solution in which the solute concentration is high and the water concentration is lower than that in the cells, water will move out of the cells and the cells will shrink. Such an environment is called hypertonic. When the water concentrations and solute concentrations on the outside and within are equal (isotonic), there will be no net movement of water. One way of determining the con centration of solute particles in the cell i! s to place the tissue in a variety of osmotic environments and determine which one causes no net uptake of water. Procedure exploitation a #5 secure borer, delete potato tuber cylinders from the potato. With a razor blade, cut the cylinders into lengths of exactly 40 mm. Be sure the cut is upright to the axis of the cylinder. Cut 24 cylinders. Label six 250 ml beakers as follows: 0.0, 0.1, 0.2, 0.3,...If you want to thrum a full essay, line of battle it on our website: BestEssayCheap.com

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